Hua
H7 Waahanga Rewharewha Rewharewha Awhiowhio ELISA Kete Whakamātautau
H7 Waahanga Rewharewha Rewharewha Awhiowhio ELISA Kete Whakamātautau
| Whakarāpopototanga | Ko te kete AIV-H7 antibody ELISA e whakamahia ana ki te kite i nga momo paturopi H7 Rewharewha Avian i roto i te serum. |
| Tikanga | Ka whakamahia e te kete whakamatautau AIV-H7 antibody ELISA ki te kimi i nga momo paturopi H7 Rewharewha Avian i roto i te serum, mo te aro turuki i te paturopi i muri i te mate AIV-H7 me te tātaritanga serological o te mate o Avian |
| Nga Whainga Rapu | AIV-H7 antibody |
| Tauira | Serum
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| Te nui | 1 kete = 192 Whakamatau |
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Te Pumau me te Rokiroki | 1) Me penapena nga reagents katoa ki te 2 ~ 8 ℃.Kaua e whakatio. 2) He 12 marama te roa o te ora.Whakamahia nga reagents katoa i mua i te ra paunga o te kete.
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Nga korero
Ko te rewharewha manu, e mohiotia opakitia ana ko te rewharewha manu, he rewharewha manu ranei, he momo rewharewha na nga wheori kua urutau kimanu.
Ko te momo tino morearea ko te rewharewha avian tino mate (HPAI).He rite te rewharewha manu ki terewharewha poaka, rewharewha kuri, rewharewha hoiho me
ko te rewharewha tangata he mate na te rewharewha o te rewharewha kua uru ki tetahi kaihautu motuhake.
O nga momo wheori rewharewha e toru (A,B, aC), rewharewha A ko te huaketo he azoonoticte mate ki te puna taiao tata
i roto i nga manu katoa. Ko te rewharewha Avian, mo te nuinga o nga kaupapa, e pa ana ki te huaketo rewharewha A.
Te Kaupapa o te Whakamatau
Ka whakamahia e tenei kete te tikanga whakataetae ELISA ki te whakakikorua i mua i nga antigens AIV-H7 i runga i nga puna microplate.I te wa e whakamatautau ana, taapirihia he tauira serum waimeha me te whaariki e tohuhia ana he anti-AIV-H7 antibody monoclonal, i muri i te incubation, ki te mea he AIV-H7 antibody, ka hono ki te antigen kua pania-mua, antibody i roto i te tauira poraka te huinga o te antibody monoclonal me te mua. - antigen paninga;makahia te conjugate whākōkī kore i whakakotahi me te horoi;Taapirihia te tïpako TMB ki roto i nga puna moroiti, ko te tohu kikorangi na te Enzyme catalysis kei roto i te owehenga rerekee o te ihirangi antibody i roto i te tauira.
Ihirangi
| Reagent | Rōrahi 96 Whakamātautau/192Whakamātautau | ||
| 1 |
| 1ea/2ea | |
| 2 |
| 2.0ml | |
| 3 |
| 1.6ml | |
| 4 |
| 100ml | |
| 5 |
| 100ml | |
| 6 |
| 11/22ml | |
| 7 |
| 11/22ml | |
| 8 |
| 15ml | |
| 9 |
| 2ea/4ea | |
| 10 | microplate dilution serum | 1ea/2ea | |
| 11 | Tohutohu | 1 pcs |
