Hua
Eggs Drop Syndrome1976 huaketo Antibody ELISA Ki
Eggs Drop Syndrome1976 huaketo Antibody ELISA Ki
| Whakarāpopototanga | Ukia kitea he antibody motuhake ki te EDS76 i roto i te serum ma te tohu. |
| Tikanga | Ko te Eggs Drop Syndrome 1976 huaketo(EDS76) Ab Elisa kete e whakamahia ana ki te kite i te paturopi motuhake ki te EDS76 i roto i te serum i te kounga.Mo te aro turuki i te antibody i muri i te EDS76 te aukati me te taatai serological o te mate i roto i te Avian. |
| Nga Whainga Rapu | Antibody ki te EDS76 i roto i te serum |
| Tauira | Serum
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| Te nui | 1 kete = 192 Whakamatau |
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Te Pumau me te Rokiroki | 1) Me penapena nga reagents katoa ki te 2 ~ 8 ℃.Kaua e whakatio. 2) He 12 marama te roa o te ora.Whakamahia nga reagents katoa i mua i te ra paunga o te kete.
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Nga korero
Ko te Egg Drop Syndrome (EDS-76) he mate hopuhopu whakapeka na te roopu adenovirus III o te puninga Adenoviridae Avian Virus me te hemagglutination.I etahi o nga paamu heihei, ka heke ohorere te maha o nga hua o nga heihei, ka puta nga hua hee penei i nga hua anga-ngohengohe, nga hua anga-kore, me nga hua anga angiangi i te wa kotahi.Ko te katoa o te mate ka roa mo te 5-6 wiki, ka mutu ka piki haere te reiti hua hua, engari he uaua ki te eke ki te taumata i mua i te heke.
Te Kaupapa o te Whakamatau
Ka whakamahia e tenei kete te tikanga ELISA autaki, ko te antigen EDS76 kua purea kua pania-mua ki runga i nga waahanga moroiti-puna enaaki.,ki te mea he EDS76virusspecificantibody, ka hono ki te antigen kua pania-mua, ka whakakorehia te paturopi kore whakakotahi me etahi atu waahanga me te horoi;kātahi ka tāpirihia te whākōkī anti-EDS76 huaketo monoclonal antibody, kātahi te whakakotahitanga o te antibody monoclonal me te antigen kua pania-mua;makahia te conjugate whākōkī kore i whakakotahi me te horoi;Taapirihia te tïpako TMB ki roto i nga puna moroiti, ko te tohu kikorangi na te Enzyme catalysis kei roto i te owehenga rerekee o te ihirangi antibody i roto i te tauira, whakamahia te kaipanui ELISA i te 450nm ngaru ngaru hei ine i te uara uru A i roto i nga puna tauhohenga i muri i te taapiri otinga mutu.
ki te whakamutu i te tauhohenga.
Ihirangi
| Reagent | Rōrahi 96 Whakamātautau/192Whakamātautau | ||
| 1 |
| 1ea/2ea | |
| 2 |
| 2.0ml | |
| 3 |
| 1.6ml | |
| 4 |
| 100ml | |
| 5 |
| 100ml | |
| 6 |
| 11/22ml | |
| 7 |
| 11/22ml | |
| 8 |
| 15ml | |
| 9 |
| 2ea/4ea | |
| 10 | microplate dilution serum | 1ea/2ea | |
| 11 | Tohutohu | 1 pcs |
