Hua
Ko te kete ELISA mate mate Newcastle
Ko te kete ELISA mate mate Newcastle
| Whakarāpopototanga | Te kitenga o nga Antibody motuhake o nga mate o Newcastle motuhake |
| Tikanga | Ka whakamahia te kete pareketo mate Newcastle Elisa ki te kite i te paturopi motuhake ki te mate Newcastle Virus (NDV) i roto i te serum, mo te aro turuki i te paturopi i muri i te aukati NDVme te tātaritanga serological o te mate i roto i te Avian.
|
| Nga Whainga Rapu | Te mate pukupuku o Newcastle |
| Tauira | Serum
|
| Te nui | 1 kete = 192 Whakamatau |
|
Te Pumau me te Rokiroki | 1) Me penapena nga reagents katoa ki te 2 ~ 8 ℃.Kaua e whakatio. 2) He 12 marama te roa o te ora.Whakamahia nga reagents katoa i mua i te ra paunga o te kete.
|
Nga korero
Te mate Newcastle he mate avian horapa e pa ana ki te maha o nga momo manu mohoao me te mohoao;ka tukuna ki te tangata.Ahakoa ka pangia e te tangata, ko te nuinga o nga keehi kaore he tohu;he uaua ka puta he kirika ngawari me nga tohu rewharewha me te conjunctivitis i roto i te tangata.Ko ona paanga ka tino kitea i roto i nga heihei o te kainga na te kaha o te whakaraerae me te kaha o nga paanga kino o te epizootic ki nga umanga heihei.He mate ki nga whenua maha.Karekau he rongoa mo tera e mohiotia ana, engari ma te whakamahi i nga kano kano aukati me nga tikanga parukore ka whakaitihia te tupono o te pakarutanga.
Te Kaupapa o te Whakamatau
Ka whakamahia e tenei kete te tikanga paraka ELISA, ko te antigen NDV kua pania-mua ki runga i te microplate.I te wa e whakamatautau ana, taapirihia he tauira serum waimeha, i muri i te hekitanga, mena he antibody motuhake NDV, ka whakakotahi ki te antigen kua pania-mua, ka makahia te antibody uncombined me etahi atu waahanga me te horoi;kātahi ka tāpirihia te whākōkī anti-NDV monoclonal antibody, antibody i roto i te tauira poraka te whakakotahitanga o te antibody monoclonal me te antigen pania-mua;makahia te conjugate whākōkī kore i whakakotahi me te horoi. Taapirihia te tïpako TMB ki roto i nga puna moroiti, ko te tohu kikorangi na te Enzyme catalysis kei roto i te owehenga rerekee o te ihirangi antibody i roto i te tauira.
Ihirangi
| Reagent | Rōrahi 96 Whakamātautau/192Whakamātautau | ||
| 1 |
| 1ea/2ea | |
| 2 |
| 2.0ml | |
| 3 |
| 1.6ml | |
| 4 |
| 100ml | |
| 5 |
| 100ml | |
| 6 |
| 11/22ml | |
| 7 |
| 11/22ml | |
| 8 |
| 15ml | |
| 9 |
| 2ea/4ea | |
| 10 | microplate dilution serum | 1ea/2ea | |
| 11 | Tohutohu | 1 pcs |
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