Hua
Heihei Infectious Bursal mate Virus Ab Elisa kete
Heihei Infectious Bursal mate Virus Ab Elisa kete
| Whakarāpopototanga | Te kitenga o te whakakore i te antibody ki te bursa hopuhopu o te huaketo Fabricius i te serum heihei |
| Nga Whainga Rapu | Ko te heihei infectious bursal disease virus antibody |
| Tauira | Serum
|
| Te nui | 1 kete = 192 Whakamatau |
|
Te Pumau me te Rokiroki | 1) Me penapena nga reagents katoa ki te 2 ~ 8 ℃.Kaua e whakatio. 2) He 12 marama te roa o te ora.Whakamahia nga reagents katoa i mua i te ra paunga o te kete.
|
Nga korero
Ko te mate urutomo(IBD), e mohiotia ana ko te mate Gumboro, te bursitis infectious me te nephrosis avian infectious, he mate hopuhopu nui o nga taiohi.heiheime nga korukoru i puta mai i te huaketo bursal disease (IBDV), e tohuhia ana ete whakahekenga mateme te matemate i te nuinga o te 3 ki te 6 wiki te pakeke.I kitea tuatahi te mate i roto iGumboro, Delawarei te tau 1962. He mea nui te ohanga ki te umanga heihei puta noa i te ao na te kaha ake o te whakaraeraetanga ki etahi atu mate me te pokanoa kino ki te whai hua.werohanga.I roto i nga tau tata nei, kua puta mai nga riu kino kino o te IBDV (vvIBDV), e mate kino ana ki te heihei, i Uropi,Amerika Latina,Ahia ki te tonga-rawhiti, Awherika me teMiddle East.Ka pangia te mate ma te ara oro-fecal, me nga manu kua pa ki te whakaputa i nga taumata teitei o te huaketo mo te 2 wiki i muri i te mate.Ka ngawari te horapa o te mate mai i nga heihei kua pangia ki nga heihei hauora ma te kai, te wai, me te whakapiri tinana.
Te Kaupapa o te Whakamatau
Ka whakamahia e te kete he tikanga whakataetae ELISA, he rongoa VP2 te huaketo bursal infectious i mua i te kete i runga i te microplate, ka whakataetae ki te anti-VP2 antibody pūmua i roto i te serum mo te vector wahanga totoka ma te whakamahi i te anti-VP2 pūmua monoclonal antibody.I roto i te whakamatautau, he antibody monoclonal hei whakamatautau me te taapiri i te pūmua anti-VP2, a, i muri i te hekitanga, mena kei roto i te tauira te heihei huaketo bursal mate huaketo VP2 pūmua-motuhake, ka herea ki te antigen i runga i te pereti pani.Na reira ka aukati i te herenga o te anti-VP2 pūmua monoclonal antibody ki te antigen, i muri i te horoinga ki te tango i te antibody unbound me etahi atu waahanga;katahi ka taapirihia he anti-kiore e tohu ana i te paturopi tuarua hei herea ki te matatini antigen-antibody i runga i te pereti rapu;Ka tangohia te conjugate enzyme kore i herea ma te horoi;ka taapirihia te TMB substrate ki te microwell hei whakawhanake i te tae, a ko te uara whakauru o te tauira ka hono kino ki te ihirangi o te anti-VP2 antibody pūmua kei roto, na reira ka tutuki te kaupapa o te kimi i te anti-VP2 pūmua antibody i roto i te tauira.
Ihirangi
| Reagent | Rōrahi 96 Whakamātautau/192Whakamātautau | ||
| 1 |
| 1ea/2ea | |
| 2 |
| 2.0ml | |
| 3 |
| 1.6ml | |
| 4 |
| 100ml | |
| 5 |
| 100ml | |
| 6 |
| 11/22ml | |
| 7 |
| 11/22ml | |
| 8 |
| 15ml | |
| 9 |
| 2ea/4ea | |
| 10 | microplate dilution serum | 1ea/2ea | |
| 11 | Tohutohu | 1 pcs |
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