Hua
Kete ELISA Antibody Rewharewha Avian
Kete ELISA Antibody Rewharewha Avian
| Whakarāpopototanga | Ka whakamahia ki te kite i nga paturopi motuhake ki te huaketo rewharewha avian (AIV) i roto i te serum |
| Tikanga | Ka whakamahia te kete a Elisa rewharewha rewharewha ki te kite i te paturopi motuhake ki te Virus rewharewha Avian (AIV) i roto i te serum, mo te aro turuki i te paturopi i muri i te mate AIV me te tātaritanga serological o te mate i Avian. |
| Nga Whainga Rapu | Antibody Rewharewha Avian |
| Tauira | Serum
|
| Te nui | 1 kete = 192 Whakamatau |
|
Te Pumau me te Rokiroki | 1) Me penapena nga reagents katoa ki te 2 ~ 8 ℃.Kaua e whakatio. 2) He 12 marama te roa o te ora.Whakamahia nga reagents katoa i mua i te ra paunga o te kete.
|
Nga korero
Ko te rewharewha manu, e mohiotia opakitia ana ko te rewharewha manu, he rewharewha manu ranei, he momo rewharewha na nga wheori kua urutau kimanu.
Ko te momo tino morearea ko te rewharewha avian tino mate (HPAI).He rite te rewharewha manu ki terewharewha poaka, rewharewha kuri, hoiho
rewharewha me te rewharewha tangata he mate na te rewharewha o te rewharewha kua urutau ki tetahi momo mate
manaaki.O nga momo wheori rewharewha e toru (A,B, aC), rewharewha A ko te huaketo he azoonoticmate ki te taiao
te puna wai tata katoa i roto i nga manu.Ko te rewharewha Avian, mo te nuinga o nga kaupapa, e pa ana ki te rewharewha A.
Te Kaupapa o te Whakamatau
Ka whakamahia e tenei kete te tikanga paraka ELISA, ko te antigen AIV kua pania i mua i runga i te microplate.I te wa e whakamatautau ana, taapirihia he tauira serum waimeha, i muri i te hekitanga, mena he AIV motuhake te antibody, ka uru ki te antigen kua pania-mua, ka makahia te antibody uncombined me etahi atu waahanga me te horoi;kātahi ka tāpirihia te whākōkī anti-AIV monoclonal antibody, antibody i roto i te tauira poraka te huinga o te antibody monoclonal me te antigen pania-mua;makahia te conjugate whākōkī kore i whakakotahi me te horoi.Taapirihia te tïpako TMB ki roto i nga puna moroiti, ko te tohu kikorangi na te Enzyme catalysis kei roto i te owehenga rerekee o te ihirangi antibody i roto i te tauira.
Ihirangi
| Reagent | Rōrahi 96 Whakamātautau/192Whakamātautau | ||
| 1 |
| 1ea/2ea | |
| 2 |
| 2.0ml | |
| 3 |
| 1.6ml | |
| 4 |
| 100ml | |
| 5 |
| 100ml | |
| 6 |
| 11/22ml | |
| 7 |
| 11/22ml | |
| 8 |
| 15ml | |
| 9 |
| 2ea/4ea | |
| 10 | microplate dilution serum | 1ea/2ea | |
| 11 | Tohutohu | 1 pcs |
