Whakarāpopototanga | Te kitenga o te mate pukupuku Bovine Tuberculosis(BTB). |
Tikanga | Ko te Bovine Tuberculosis(BTB) antibody Elisa ka taea te whakamahi kete whakamatautau ki te kite i te Bovine Tuberculosis antibody i roto i te serum, plasma ranei o Bovine. |
Nga Whainga Rapu | Bovine Tuberculosis(BTB) antibody |
Tauira | Serum
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Te nui | 1 kete = 192 Whakamatau |
Te Pumau me te Rokiroki | 1) Me penapena nga reagents katoa ki te 2 ~ 8 ℃.Kaua e whakatio. 2) 12 marama te roa o te ora.Whakamahia nga reagents katoa i mua i te ra paunga o te kete.
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Ko te Mycobacterium bovis he puhoi te tipu (16- ki te 20-haora te wa whakatipuranga) huakita aerobic me te kaikawe o te mate kohi i roto i nga kau (e mohiotia ana ko te TB bovine).E pa ana ki te Mycobacterium tuberculosis, te huakita e puta ai te mate kohi i roto i te tangata.Ka taea e M. bovis te peke i te arai momo ka pa mai te mate kohi-rite ki te tangata me etahi atu kararehe whakangote.
Tenei kete whakamahi autaki ELISA tikanga, parakore BTB antigen is pani-mua on whākōkī moroiti-puna takai. A, no te whakamatautau, taapiri diluted serum tauira, muri heki, if i reira is BTB motuhake antibody, it hiahia whakakotahi me te pani-mua antigen, whakarerea te kore whakakotahi antibody a atu nga waahanga me horoi; katahi tāpiri whākōkī whakakotahi, whakarerea te kore whakakotahi whākōkī whakakotahi me
horoi. Taapirihia te tïpako TMB ki roto i nga puna moroiti, he tika te tohu kikorangi na te Enzyme catalysis te wahanga o te ihirangi antibody i roto i te tauira.
Reagent | Rōrahi 96 Whakamātautau/192Whakamātautau | ||
1 |
| 1ea/2ea | |
2 |
| 2.0ml | |
3 |
| 1.6ml | |
4 |
| 100ml | |
5 |
| 100ml | |
6 |
| 11/22ml | |
7 |
| 11/22ml | |
8 |
| 15ml | |
9 |
| 2ea/4ea | |
10 | microplate waimehatanga serum | 1ea/2ea | |
11 | Tohutohu | 1 pcs |