Whakarāpopototanga | Te kitenga o te Antibody motuhake o Peste Des Petits Ruminants |
Tikanga | Ka whakamahia e te kete whakamatautau ELISA PPRV antibody ki te kite i nga paturopi huaketo Peste des petits ruminants i roto i te serum o te hipi me te koati. |
Nga Whainga Rapu | PPRV antibody |
Tauira | Serum
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Te nui | 1 kete = 192 Whakamatau |
Te Pumau me te Rokiroki | 1) Me penapena nga reagents katoa ki te 2 ~ 8 ℃.Kaua e whakatio. 2) 12 marama te roa o te ora.Whakamahia nga reagents katoa i mua i te ra paunga o te kete.
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Ovine rinderpest, e mohiotia ana anopeste des petits ruminants(PPR), he mate hopuhopu e pa anakoatiahipi;heoi, ko nga kamera me nga mohoao itiruminantska taea hoki te pa.PPR kei roto i tenei waTe Taitokerau, Pokapū, Te uruaAwherika ki te Rawhiti, teMiddle East, aAhia ki te Tonga. Na teruminants iti morbillivirusi roto i te puningaMorbilivirus,a he hononga tata ki etahi atu, rinderpest morbillivirus, mate karawaka morbillivirus, amorbilivirus kuri(i mohiotia i mua kokurihuaketo distemper).He tino hopuhopu te mate, a ka taea te 80-100% te mate mate ki rotowhakapekatake i roto i teepizooticwhakatakotoranga.Kare te huaketo e pangia e te tangata.
Ko te PPR e mohiotia ana ko te whiu koati,kata, te mate o te stomatitis-pneumoenteritis, me te ovine rinderpest.
Nga tari whai mana penei i teFAOaOIEwhakamahia te ingoa French "peste des petits ruminants" me te maha o nga momo tuhi.
Ka whakamahia e tenei kete te tikanga whakataetae ELISA ki te whakakikorua i mua i nga antigens PPRV i runga i nga puna moroiti.A, no te whakamatautau, tāpiri tauira serum diluted, i muri heki, ki te he PPRV antibody, ka paheko reira ki te antigen pani-mua, antibody i roto i te tauira poraka te huinga o te antibody monoclonal me te antigen pani-mua;makahia te conjugate whākōkī kore i whakakotahi me te horoi;Taapirihia te tïpako TMB ki roto i nga puna moroiti, ko te tohu kikorangi na te Enzyme catalysis kei roto i te owehenga rerekee o te ihirangi antibody i roto i te tauira.
Reagent | Rōrahi 96 Whakamātautau/192Whakamātautau | ||
1 |
| 1ea/2ea | |
2 |
| 2ml | |
3 |
| 1.6ml | |
4 |
| 100ml | |
5 |
| 100ml | |
6 |
| 11/22ml | |
7 |
| 11/22ml | |
8 |
| 15ml | |
9 |
| 2ea/4ea | |
10 | microplate waimehatanga serum | 1ea/2ea | |
11 | Tohutohu | 1 pcs |