Whakarāpopototanga | Te kitenga o te Antibody of Flu He tohu mate me te serological o te mate i roto i te Avian, swine me Equus. |
Tikanga | Rewharewha Ka whakamahia te kete a Elisa hei whakamohio i te paturopi motuhake ki a Irewharewha A Virus (Rewharewha A) i roto i te serum, mo te aro turuki i te antibody i muri i te Rewharewha He aukatime te tātaritanga serological o te mate i roto i te Avian, te poaka me te Equus.
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Nga Whainga Rapu | Rewharewha A antibody |
Tauira | Serum
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Te nui | 1 kete = 192 Whakamatau |
Te Pumau me te Rokiroki | 1) Me penapena nga reagents katoa ki te 2 ~ 8 ℃.Kaua e whakatio. 2) 12 marama te roa o te ora.Whakamahia nga reagents katoa i mua i te ra paunga o te kete.
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Ko te huaketo rewharewha A he tukumate e puta ai te rewharewha ki roto i nga manu me etahi momo whakangote.He huaketo RNA, kua wehea etahi momo iti mai i nga manu mohoao.I etahi wa, ka horapa mai i nga manu mohoao ki nga heihei, tera pea ka pa ki nga mate kino, nga pakarutanga, nga mate rewharewha tangata ranei.
Ka whakamahia e tenei kete te tikanga paraka ELISA, ko te antigen FluA kua pania i mua i te pereti moroiti.I te wa e whakamatautau ana, taapirihia he tauira serum kua waimeha, i muri i te hekitanga, mena he Rewharewha He antibody motuhake, ka hono ki te antigen kua pania-mua, ka makahia te antibody uncombined me etahi atu waahanga me te horoi;kātahi ka tāpirihia te whākōkī kua tapaina he anti-Rewharewha He antibody monoclonal, antibody i roto i te tauira poraka te whakakotahitanga o te antibody monoclonal me te antigen kua pania-mua;makahia te conjugate whākōkī kore i whakakotahi me te horoi. Taapirihia te tïpako TMB ki roto i nga puna moroiti, ko te tohu kikorangi na te Enzyme catalysis kei roto i te owehenga rerekee o te ihirangi antibody i roto i te tauira.
Reagent | Rōrahi 96 Whakamātautau/192Whakamātautau | ||
1 |
| 1ea/2ea | |
2 |
| 2.0ml | |
3 |
| 1.6ml | |
4 |
| 100ml | |
5 |
| 100ml | |
6 |
| 11/22ml | |
7 |
| 11/22ml | |
8 |
| 15ml | |
9 |
| 2ea/4ea | |
10 | microplate waimehatanga serum | 1ea/2ea | |
11 | Tohutohu | 1 pcs |