Whakarāpopototanga | Te kitenga o nga Antibody motuhake o te AIV-H9 huaketo motuhake |
Tikanga | H9 Subtype Avian Rewharewha antibody Elisa ka taea te whakamahi kete whakamatautau hei kite i te H9 Subtype Avian Rewharewha antibody i roto i te serum o te heihei.
|
Nga Whainga Rapu | H9 Subtype Antibody Rewharewha Avian |
Tauira | Serum
|
Te nui | 1 kete = 192 Whakamatau |
Te Pumau me te Rokiroki | 1) Me penapena nga reagents katoa ki te 2 ~ 8 ℃.Kaua e whakatio. 2) 12 marama te roa o te ora.Whakamahia nga reagents katoa i mua i te ra paunga o te kete.
|
Ko te rewharewha manu, e mohiotia opakitia ana ko te rewharewha manu, he rewharewha manu ranei, he momo rewharewha na nga wheori kua urutau kimanu.Ko te momo me te tino tupono he tino nui
rewharewha avian pathogenic (HPAI).He rite te rewharewha manu ki terewharewha poaka, rewharewha kuri, rewharewha hoiho me te rewharewha tangata he mate na te rewharewha rewharewha e pa ana
ka urutau ki tetahi kaihautu motuhake.O nga momo wheori rewharewha e toru (A,B, aC), ko te rewharewha A he huaketo hezoonoticte mate ki te puna taiao tata
i roto i nga manu katoa. Ko te rewharewha Avian, mo te nuinga o nga kaupapa, e pa ana ki te huaketo rewharewha A.
Tenei kete whakamahi autaki ELISA tikanga, parakore AIV-H9HA antigen is pani-mua on whākōkī moroiti-puna takai. A, no te whakamatautau, taapiri diluted serum tauira, muri heki, if i reira is AIV-H9 huaketo motuhake antibody, it hiahia whakakotahi me te pani-mua antigen, whakarerea te kore whakakotahi antibody a atu nga waahanga me horoi; katahi tāpiri whākōkī whakakotahi, whakarerea te kore whakakotahi whākōkī whakakotahi me te horoi. Taapirihia te tïpako TMB ki roto i nga puna moroiti, he tika te tohu kikorangi na te Enzyme catalysis te wahanga o te ihirangi antibody i roto i te tauira.
Reagent | Rōrahi 96 Whakamātautau/192Whakamātautau | ||
1 |
| 1ea/2ea | |
2 |
| 2.0ml | |
3 |
| 1.6ml | |
4 |
| 100ml | |
5 |
| 100ml | |
6 |
| 11/22ml | |
7 |
| 11/22ml | |
8 |
| 15ml | |
9 |
| 2ea/4ea | |
10 | microplate waimehatanga serum | 1ea/2ea | |
11 | Tohutohu | 1 pcs |