Whakarāpopototanga | i whakamahia ki te kite i te antigen leukosis Avian P27 i roto i te toto manu, te feces, te cloaca, me te ma hua manu. |
Tikanga | Avian Leukosis (AL) P27 antigen Elisa kete e whakamahia ana ki te kite i te antigen leukosis Avian P27 i roto i te toto avian, te tai, te cloaca, me te hua manu ma.
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Nga Whainga Rapu | Manukano manu (AL) P27 antigen |
Tauira | Serum
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Te nui | 1 kete = 192 Whakamatau |
Te Pumau me te Rokiroki | 1) Me penapena nga reagents katoa ki te 2 ~ 8 ℃.Kaua e whakatio. 2) 12 marama te roa o te ora.Whakamahia nga reagents katoa i mua i te ra paunga o te kete.
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Ko te Avian Leukosis (AL) he kupu huinga mo nga momo mate pukupuku e pa ana ki nga heihei na te Avian Leukosis Virus (ALV) i roto i te whanau Retroviridae.Ko tenei mate kua toha ki te ao katoa, he nui te reeti mate.Ka taea e ia te mate me te ngoikore o nga heihei, te whakaheke i te kaha o te hanga o te kahui, a ko tetahi o nga mate matua e tino whakararu ana i te whanaketanga o te umanga heihei.He roa te hitori o tenei mate, a kei te mau tonu nga keehi hou, penei i te Avian leukemia virus subgroup J (ALV-J), i kitea, i kitea i te mutunga o nga tau 1980 i te UK he momo iti hou o te huaketo leukemia avian, he tino nui. he kino ki te ahumahi broiler
Ka whakamahia e te kete te tikanga ELISA hanawiti, kua whakakikoruatia i mua i te anti-avian leukocyte P27 monoclonal antibody ki runga i nga waahanga moroiti. te antigen unbound and other parts, te enzyme monoclonal antibody ka taapirihia kia tino herea ki te matatini antigen-antibody i runga i te pereti whakamatautau.ka horoi, ka tangohia te conjugate whākōkī kore i herea, ka tāpirihia te otinga tïpako TMB ki te microplate, ko te tohu kikorangi na te Enzyme catalysis he tika te owehenga o te ihirangi antibody i roto i te tauira.Taapirihia he otinga mutu, I muri i te tauhohenga, ka ine te uara A i roto i te puna tauhohenga ma te roangaru o te 450 nm.
Reagent | Rōrahi 96 Whakamātautau/192Whakamātautau | ||
1 |
| 1ea/2ea | |
2 |
| 2.0ml | |
3 |
| 1.6ml | |
4 |
| 100ml | |
5 |
| 100ml | |
6 |
| 11/22ml | |
7 |
| 11/22ml | |
8 |
| 15ml | |
9 |
| 2ea/4ea | |
10 | microplate waimehatanga serum | 1ea/2ea | |
11 | Tohutohu | 1 pcs |