Hua
H5 Subtype Rewharewha Rewharewha Rewharewha ELISA Kit
H5 Subtype Rewharewha Rewharewha Rewharewha ELISA Kit
| Whakarāpopototanga | Used ki te kitea motuhake antibody ki te Virus rewharewha Avian (AIV-H5) i te serum |
| Tikanga | H5 momo rewharewha avianantibodyKa whakamahia te kete Elisa ki te kite i te paturopi motuhakeAvian Rewharewha Virus (AIV-H5) i roto i te serum, mo te aro turuki i te antibody i muri i te aukati AIV-H5me te tātaritanga serological o te mate i roto i te Avian.
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| Nga Whainga Rapu | H5 subtype rewharewha avian antibody |
| Tauira | Serum
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| Te nui | 1 kete = 192 Whakamatau |
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Te Pumau me te Rokiroki | 1) Me penapena nga reagents katoa ki te 2 ~ 8 ℃.Kaua e whakatio. 2) 12 marama te roa o te ora.Whakamahia nga reagents katoa i mua i te ra paunga o te kete.
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Nga korero
Ko te rewharewha manu, e mohiotia opakitia ana ko te rewharewha manu, he rewharewha manu ranei, he momo rewharewha na nga wheori kua urutau kimanu.
Ko te momo tino morearea ko te rewharewha avian tino mate (HPAI).He rite te rewharewha manu ki terewharewha poaka, rewharewha kuri, rewharewha hoiho me
ko te rewharewha tangata he mate na te rewharewha o te rewharewha kua uru ki tetahi kaihautu motuhake.
O nga momo wheori rewharewha e toru (A,B, aC), ko te rewharewha A he huaketo hezoonoticte mate ki te puna taiao tata
i roto i nga manu katoa. Ko te rewharewha Avian, mo te nuinga o nga kaupapa, e pa ana ki te huaketo rewharewha A.
Te Kaupapa o te Whakamatau
Ka whakamahia e tenei kete te tikanga paraka ELISA, ko te antigen AIV-H5 kua pania i mua i runga i te microplate.I te wa e whakamatautau ana, taapirihia he tauira serum kua whakakorehia, i muri i te whakamohoatanga, mena he AIV-H5 he antibody motuhake, ka whakakotahi me te antigen kua pania-mua, ka makahia te antibody uncombined me etahi atu waahanga me te horoi;kātahi ka tāpirihia te whākōkī kua tapaina he anti-AIV-H5 monoclonal antibody, antibody i roto i te tauira poraka te huinga o te antibody monoclonal me te antigen kua pania-mua;makahia te conjugate whākōkī kore i whakakotahi me te horoi. Taapirihia te tïpako TMB ki roto i nga puna moroiti, ko te tohu kikorangi na te Enzyme catalysis kei roto i te owehenga rereke o te ihirangi antibody i roto i te tauira..
Ihirangi
| Reagent | Rōrahi 96 Whakamātautau/192Whakamātautau | ||
| 1 |
| 1ea/2ea | |
| 2 |
| 2.0ml | |
| 3 |
| 1.6ml | |
| 4 |
| 100ml | |
| 5 |
| 100ml | |
| 6 |
| 11/22ml | |
| 7 |
| 11/22ml | |
| 8 |
| 15ml | |
| 9 |
| 2ea/4ea | |
| 10 | microplate waimehatanga serum | 1ea/2ea | |
| 11 | Tohutohu | 1 pcs |
