Whakarāpopototanga | Te kitenga o nga mea motuhakeWaewae me te Mangai antibodyMomo O |
Tikanga | Ko te FMD Momo Ahia I te kete whakamatautau ELISA e whakamahia ana ki te kite i nga paturopi huaketo mate-waewae me te waha i roto i te serum o te poaka, kau, hipi me te koati mo te arotake i te mate kano kano FMD. |
Nga Whainga Rapu | Waewae me te Mangai antibodyMomo O |
Tauira | Serum
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Te nui | 1 kete = 192 Whakamatau |
Te Pumau me te Rokiroki | 1) Me penapena nga reagents katoa ki te 2 ~ 8 ℃.Kaua e whakatio. 2) 12 marama te roa o te ora.Whakamahia nga reagents katoa i mua i te ra paunga o te kete.
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Te mate wheori waewae-a-waha(FMDV) ko tetukumatee puta aimate waewae-a-waha.[1]He apicornavirus, te mema tauira o te puningaAphthovirus.Ko te mate, e puta ai nga vesicles (opupu) i roto i te waha me nga waewae okau, poaka, hipi, koati, me era atukawau-maamahe tino mate hopuhopu me te mate nui o nga karareheahuwhenua kararehe
Ko te tikanga ELISA-kore tenei, kua pania te FMDVantigeni i mua i te paninga i runga i nga papaa moroiti-wai.I te wa e whakamatautauria ana, ka taapirihia te tauira serum, i muri i te hekitanga, ki te mea he antibody motuhake te huaketo FMD, ka uru ki te antigen kua pania-mua, ka makahia te antibody uncombined me etahi atu waahanga me te horoi;kātahi ka tāpirihia te whākōkī conjugate, makahia te conjugate whākōkī kore ki te horoi.Taapirihia te tïpako TMB ki roto i nga puna moroiti, ko te tohu kikorangi na te Enzyme catalysis ko te wahanga tika o te ihirangi antibody i roto i te tauira.
Reagent | Rōrahi 96 Whakamātautau/192Whakamātautau | ||
1 |
| 1ea/2ea | |
2 |
| 2ml | |
3 |
| 1.6ml | |
4 |
| 100ml | |
5 |
| 100ml | |
6 |
| 11/22ml | |
7 |
| 11/22ml | |
8 |
| 15ml | |
9 |
| 2ea/4ea | |
10 | microplate waimehatanga serum | 1ea/2ea | |
11 | Tohutohu | 1 pcs |