Whakarāpopototanga | Te kitenga o nga mea motuhakeTe mate Waewae me te Waaha Momo A antibody |
Tikanga | Ka whakamahia e te kete whakamatautau ELISA mo te FMD Momo A antibody ki te kite i nga paturopi huaketo mate-waewae me te waha i roto i te serum o te poaka, kau, hipi me te koati hei arotake i te mate kano kano FMD. |
Nga Whainga Rapu | Te Waewae me te Waaha Momo A Antibody |
Tauira | Serum
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Te nui | 1 kete = 192 Whakamatau |
Te Pumau me te Rokiroki | 1) Me penapena nga reagents katoa ki te 2 ~ 8 ℃.Kaua e whakatio. 2) 12 marama te roa o te ora.Whakamahia nga reagents katoa i mua i te ra paunga o te kete.
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Te mate wheori waewae-a-waha(FMDV) ko tetukumatee puta aimate waewae-a-waha. He apicornavirus, te mema tauira o te puningaAphthovirus.Ko te mate, e puta ai nga vesicles (opupu) i roto i te waha me nga waewae okau, poaka, hipi, koati, me era atukawau-maamahe tino mate hopuhopu me te mate nui o nga karareheahuwhenua kararehe.
Ka whakamahia e tenei kete nga tikanga whakataetae ELISA ki te pania i mua i nga antigens wheori mate-waewae me te waha i runga i nga puna moroiti.I te wa e whakamatautau ana, taapirihia te tauira serum kua diluted me te anti-FMD Ab, i muri i te whakamohoatanga, mena he antibody FMD, ka whakakotahi me te antigen kua pania-mua, antibody i roto i te paraka tauira te whakakotahitanga o te anti-FMD antibody me te antigen pania-mua;makahia te conjugate whākōkī kore i whakakotahi me te horoi;Taapirihia te tïpako TMB ki roto i nga puna moroiti, ko te tohu kikorangi na te Enzyme catalysis kei roto i te owehenga rerekee o te ihirangi antibody i roto i te tauira.
Te mate wheori waewae-a-wahaputa i roto i whitu nuiserotypes: O, A, C, SAT-1, SAT-2, SAT-3, me Ahia-1.E whakaatu ana enei momo tohu i etahi ahuatanga o te rohe, a ko te O serotype he mea noa.
Reagent | Rōrahi 96 Whakamātautau/192Whakamātautau | ||
1 |
| 1ea/2ea | |
2 |
| 2.0ml | |
3 |
| 1.6ml | |
4 |
| 100ml | |
5 |
| 100ml | |
6 |
| 11/22ml | |
7 |
| 11/22ml | |
8 |
| 15ml | |
9 |
| 2ea/4ea | |
10 | microplate waimehatanga serum | 1ea/2ea | |
11 | Tohutohu | 1 pcs |