Whakarāpopototanga | Te kitenga o te Antibody motuhake o te Waewae me te MouthDisease Momo Te BRU |
Tikanga | Ka whakamahia e te kete whakamatautau BRU antibody ELISA ki te kite i nga paturopi Brucellosis i roto i te serum o te poaka, kau, hipi me te koati. |
Nga Whainga Rapu | Ko te BRU antibody |
Tauira | Serum
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Te nui | 1 kete = 192 Whakamatau |
Te Pumau me te Rokiroki | 1) Me penapena nga reagents katoa ki te 2 ~ 8 ℃.Kaua e whakatio. 2) 12 marama te roa o te ora.Whakamahia nga reagents katoa i mua i te ra paunga o te kete.
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Ko te BRU antibodyELISAkete whakamatautauwhakamahia ki te kitenga o nga paturopi Brucellosis i te serum o te poaka, kau, shipi me te koati .
Ka whakamahia tenei kete tikanga whakataetae ELISA ki mua i te kotied BRU antigens i runga i nga puna microplate.I te wa e whakamatautau ana, taapirihia he tauira serum kua diluted awhākōkī whakaingoa anti-BRU monoclonal antibody, i muri i te heki, mena kei reira whai BRU antibody, ka whakakotahi ki te antigen kua pania-mua, antibody i roto i te tauira poraka te huinga o te antibody monoclonal me te antigen kua pania-mua;makahia te conjugate whākōkī kore i whakakotahi me te horoi;Taapirihia te tïpako TMB ki roto i nga puna moroiti, ko te tohu kikorangi na te Enzyme catalysis kei roto i te owehenga rereke o te ihirangi antibody i roto i te tauira..
Reagent | Rōrahi 96 Whakamātautau/192Whakamātautau | ||
1 |
| 1ea/2ea | |
2 |
| 2ml | |
3 |
| 1.6ml | |
4 |
| 100ml | |
5 |
| 100ml | |
6 |
| 11/22ml | |
7 |
| 11/22ml | |
8 |
| 15ml | |
9 |
| 2ea/4ea | |
10 | microplate waimehatanga serum | 1ea/2ea | |
11 | Tohutohu | 1 pcs |